Genomics-driven discovery of a new cyclodepsipeptide from the guanophilic fungus Amphichorda guana.
Min LiangHai-Ning LyuZi-Ying MaEr-Wei LiLei CaiWen-Bing YinPublished in: Organic & biomolecular chemistry (2021)
Two potential non-ribosomal peptide synthetases (NRPSs) were identified in the genome of a guanophilic fungus Amphichorda guana by bioinformatics analysis and gene knockout experiments. Liquid chromatography coupled with mass spectrometry (LC-MS) guided isolation led to the discovery of a new cyclodepsipeptide isaridin H (1) and seven known analogs, desmethylisaridin E (2), isaridin E (3), isariin A (4), iso-isariin B (5), iso-isariin D (6), isariin E (7), and nodupetide (8). The absolute configuration of isaridin H (1) was achieved by Marfey's method. Isaridin H (1) showed significant antifungal activity against Botrytis cinerea and Alternaria solani.
Keyphrases
- liquid chromatography
- mass spectrometry
- bioinformatics analysis
- small molecule
- high resolution mass spectrometry
- tandem mass spectrometry
- high throughput
- genome wide
- high performance liquid chromatography
- gas chromatography
- single cell
- simultaneous determination
- solid phase extraction
- high resolution
- copy number
- molecular docking
- gene expression
- human health
- risk assessment
- transcription factor
- climate change
- genome wide analysis