Self-assembly of protein-DNA superstructures for alkaline phosphatase detection in blood.

Yangyang ChangQian ZhangWei XueYanfang WuYi LiuMeng Liu

Published in: Chemical communications (Cambridge, England) (2023)

We designed a paper-based analytical device by integrating horseradish peroxidase (HRP)-encapsulated 3D DNA for visual detection of alkaline phosphatase (ALP). This device allows on-paper sample pre-treatment, target recognition and signal readout, enabling simple (without additional pre-treatment of blood samples) and rapid (within 23 min) determination of ALP in clinical samples.

Keyphrases

loop mediated isothermal amplification
circulating tumor
single molecule
hydrogen peroxide
small molecule
real time pcr
mass spectrometry
liquid chromatography
solid phase extraction
tandem mass spectrometry