Early and Late Effects of Low-Dose X-ray Exposure in Human Fibroblasts: DNA Repair Foci, Proliferation, Autophagy, and Senescence.
Andrey OsipovAnna ChigasovaElizaveta YashkinaMaxim IgnatovNatalia VorobyevaNikolay ZyuzikovAndreyan N OsipovPublished in: International journal of molecular sciences (2024)
The effects of low-dose radiation exposure remain a controversial topic in radiation biology. This study compares early (0.5, 4, 24, 48, and 72 h) and late (5, 10, and 15 cell passages) post-irradiation changes in γH2AX, 53BP1, pATM, and p-p53 (Ser-15) foci, proliferation, autophagy, and senescence in primary fibroblasts exposed to 100 and 2000 mGy X-ray radiation. The results show that exposure to 100 mGy significantly increased γH2AX, 53BP1, and pATM foci only at 0.5 and 4 h post irradiation. There were no changes in p-p53 (Ser-15) foci, proliferation, autophagy, or senescence up to 15 passages post irradiation at the low dose.
Keyphrases
- low dose
- signaling pathway
- dna damage
- dna repair
- endothelial cells
- cell death
- endoplasmic reticulum stress
- oxidative stress
- high dose
- radiation induced
- high resolution
- stress induced
- extracellular matrix
- dna damage response
- single cell
- cell therapy
- radiation therapy
- stem cells
- magnetic resonance
- computed tomography
- electron microscopy