Login / Signup

Critical Role of Monooxygenase in Biodegradation of 2,4,6-Trinitrotoluene by Buttiauxella sp. S19-1.

Miao XuLei HePing SunMing WuXiyan CuiDong LiuAmma G Adomako-BonsuMin GengGuangming XiongLiquan GuoEdmund Maser
Published in: Molecules (Basel, Switzerland) (2023)
2,4,6-Trinitrotoluene (TNT) is an aromatic pollutant that is difficult to be degraded in the natural environment. The screening of efficient degrading bacteria for bioremediation of TNT has received much attention from scholars. In this paper, transcriptome analysis of the efficient degrading bacterium Buttiauxella sp. S19-1 revealed that the monooxygenase gene ( BuMO ) was significantly up-regulated during TNT degradation. S-Δ MO (absence of BuMO gene in S19-1 mutant) degraded TNT 1.66-fold less efficiently than strain S19-1 (from 71.2% to 42.9%), and E- MO mutant ( Escherichia coli BuMO -expressing strain) increased the efficiency of TNT degradation 1.33-fold (from 52.1% to 69.5%) for 9 h at 180 rpm at 27 °C in LB medium with 1.4 µg·mL -1 TNT. We predicted the structure of BuMO and purified recombinant BuMO (rBuMO). Its specific activity was 1.81 µmol·min -1 ·mg -1 protein at pH 7.5 and 35 °C. The results of gas chromatography mass spectrometry (GC-MS) analysis indicated that 4-amino-2,6-dinitrotoluene (ADNT) is a metabolite of TNT biodegradation. We speculate that MO is involved in catalysis in the bacterial degradation pathway of TNT in TNT-polluted environment.
Keyphrases
  • escherichia coli
  • gas chromatography mass spectrometry
  • gene expression
  • single cell
  • copy number
  • risk assessment
  • amino acid
  • dna methylation
  • high resolution
  • multidrug resistant
  • protein protein
  • genome wide analysis