Eleven oleanane-type triterpenoids named soyasapogenols B1-B11 have been obtained unexpectedly from a marine actinomycete Nonomuraea sp. MYH522. Their structures have been determined by extensive analysis of spectroscopic experiments and X-ray crystallographic data. Soyasapogenols B1-B11 exhibit subtle differences in the positions and degrees of oxidation on an oleanane skeleton. The feeding experiment suggested that soyasapogenols might be derived from soyasaponin Bb through microbial-mediated conversion. The biotransformation pathways from soyasaponin Bb to five oleanane-type triterpenoids and six A-ring cleaved analogues were proposed. The assumed biotransformation involves an array of reactions including regio- and stereo-selective oxidation. These compounds alleviated the 5,6-dimethylxanthenone-4-acetic acid-induced inflammation in Raw264.7 cells via the stimulator of interferon genes/TBK1/NF-κB signaling pathway. The present work provided an efficient approach for rapid diversification of soyasaponins and for developing food supplements with potent anti-inflammatory effects.
Keyphrases
- signaling pathway
- induced apoptosis
- anti inflammatory
- high resolution
- oxidative stress
- microbial community
- pi k akt
- molecular docking
- growth factor
- hypertrophic cardiomyopathy
- hydrogen peroxide
- cell cycle arrest
- dendritic cells
- epithelial mesenchymal transition
- genome wide
- magnetic resonance imaging
- endoplasmic reticulum stress
- high throughput
- heart failure
- mass spectrometry
- magnetic resonance
- high density
- computed tomography
- sensitive detection
- atrial fibrillation
- nuclear factor
- deep learning
- molecular dynamics simulations
- left ventricular
- single cell
- artificial intelligence
- genome wide analysis