Epigenetic Modifiers Affect the Bioactive Compounds Secreted by an Endophyte of the Tropical Plant Piper longum.
Fuad AmeenAbobakr AlmansobMona Al TamiNouf Mohammed Al-EnaziAhmed Al-SabriRaha OrfaliPublished in: Molecules (Basel, Switzerland) (2020)
Seven endophytic fungi were isolated from the tropical medicinal plant Piper longum L. After preliminary screening, Phomopsis heveicola was selected for the epigenetic activation treatments. The antibacterial, antifungal, and antioxidant potentials of crude extracts obtained from the treatments (with and without epigenetic modifiers) were analyzed in vitro. The extracts inhibited growth of the human pathogens Pseudomonas aeruginosa, Shigella sonnei, Streptococcus pyogenes, and Salmonella typhi, as well as the phytopathogens Puccinia recondita, Rhizoctonia solani, Phytophthora infestans, and Botrytis cinerea. Furthermore, DPPH-scavenging activity was higher in valproic acid treated extracts. Volatile chemicals with known biological activities (measured with GC-MS/MS), were released in the valproic acid treatment. The antimicrobial potentials of the extracts were confirmed using MRM/MS analysis. The experiments revealed a new promising endophytic fungus, P. heveicola, to be utilized in biological plant protection and in biomedical applications.
Keyphrases
- ms ms
- dna methylation
- pseudomonas aeruginosa
- gene expression
- candida albicans
- climate change
- biofilm formation
- cystic fibrosis
- mass spectrometry
- essential oil
- staphylococcus aureus
- escherichia coli
- anti inflammatory
- gas chromatography
- cell wall
- liquid chromatography tandem mass spectrometry
- newly diagnosed
- acinetobacter baumannii
- multidrug resistant
- data analysis