Quantification of citrullinated histones: Development of an improved assay to reliably quantify nucleosomal H3Cit in human plasma.
Charlotte ThålinKatherina AguileraNathan W HallMatthew R MarundeJonathan M BurgAxel RosellMaud DaleskogMaja MånssonYohei HisadaMatthew J MeinersZu-Wen SunMatthew F WhelihanMarcus A CheekSarah A HowardShruti Saxena-BeemDenis F NoubouossieNigel S KeySaira Z SheikhMichael-Christopher KeoghMartis W CowlesStaffan LundströmNigel MackmanHåkan WallénAndrea L JohnstonePublished in: Journal of thrombosis and haemostasis : JTH (2020)
Our novel approach using defined nucleosome controls enables reliable quantification of H3Cit in human plasma. This assay will be broadly applicable to study the role of histone citrullination in disease and its utility as a biomarker.