Molecular detection of multidrug and colistin-resistant Escherichia coli isolated from house flies in various environmental settings.
Abdus SoburZobayda F HaqueAbdullah Am SabujSamina IevyAmm Taufiqur RahmanMohamed Ezzat El ZowalatyMd Tanvir RahmanPublished in: Future microbiology (2019)
Aim: To detect antibiotic-resistant pathogenic Escherichia coli in house flies captured from various environmental settings. Materials & methods: Isolation and identification of E. coli was performed based on culture and PCR. Antimicrobial susceptibility testing was conducted using disk diffusion test. Detection of virulence and antimicrobial resistance genes was carried out using PCR methods. Results: The prevalence of E. coli in flies was 61% with highest in dairy farms (70.67%) followed by food centers (65.33%), dustbins (64%) and area close to hospital (44%). The prevalence rates of tetA, tetB, ereA, CITM, SHV, mcr-3 and catA1 resistance determinants were 88.75, 48.52, 41.67, 37.17, 27.77, 18.37 and 14.81%, respectively. Conclusion: Multidrug-resistant pathogenic E. coli are widely distributed in house flies, which requires a one-health approach to delineate their control.
Keyphrases
- escherichia coli
- antimicrobial resistance
- drosophila melanogaster
- klebsiella pneumoniae
- multidrug resistant
- human health
- risk factors
- biofilm formation
- healthcare
- real time pcr
- drug resistant
- public health
- risk assessment
- mental health
- genome wide
- life cycle
- staphylococcus aureus
- loop mediated isothermal amplification
- dna methylation
- gene expression
- acute care
- emergency department
- adverse drug
- social media
- quantum dots