Proteomics in the World of Induced Pluripotent Stem Cells.
Rafael Soares LindosoTais H Kasai-BrunswickGustavo MonneratFederica CollinoAdriana Bastos CarvalhoAntonio Carlos Campos de CarvalhoAdalberto VieyraPublished in: Cells (2019)
Omics approaches have significantly impacted knowledge about molecular signaling pathways driving cell function. Induced pluripotent stem cells (iPSC) have revolutionized the field of biological sciences and proteomics and, in particular, has been instrumental in identifying key elements operating during the maintenance of the pluripotent state and the differentiation process to the diverse cell types that form organisms. This review covers the evolution of conceptual and methodological strategies in proteomics; briefly describes the generation of iPSC from a historical perspective, the state-of-the-art of iPSC-based proteomics; and compares data on the proteome and transcriptome of iPSC to that of embryonic stem cells (ESC). Finally, proteomics of healthy and diseased cells and organoids differentiated from iPSC are analyzed.
Keyphrases
- induced pluripotent stem cells
- mass spectrometry
- label free
- single cell
- embryonic stem cells
- signaling pathway
- induced apoptosis
- healthcare
- gene expression
- rna seq
- oxidative stress
- electronic health record
- big data
- machine learning
- epithelial mesenchymal transition
- mesenchymal stem cells
- cell therapy
- cell proliferation
- gram negative