A DNA-Based Assay for Digoxin Detection.
Michael V KjelstrupLine D F NielsenMalthe Hansen-BruhnKurt Vesterager GothelfPublished in: Biosensors (2018)
The most common method for quantifying small-molecule drugs in blood samples is by liquid chromatography in combination with mass spectrometry. Few immuno-based assays are available for the detection of small-molecule drugs in blood. Here we report on a homogeneous assay that enables detection of the concentration of digoxin spiked into in a plasma sample. The assay is based on a shift in the equilibrium of a DNA strand displacement competition reaction, and can be performed in 30 min for concentrations above 10 nM. The equilibrium shift occurs upon binding of anti-digoxigenin antibody. As a model, the assay provides a potential alternative to current small-molecule detection methods used for therapeutic drug monitoring.
Keyphrases
- small molecule
- high throughput
- mass spectrometry
- liquid chromatography
- loop mediated isothermal amplification
- label free
- real time pcr
- protein protein
- molecular dynamics
- high resolution
- molecular dynamics simulations
- single molecule
- risk assessment
- drug induced
- ms ms
- cell free
- nucleic acid
- human health
- electron transfer