Login / Signup

Characterization of neutralizing antibody with prophylactic and therapeutic efficacy against SARS-CoV-2 in rhesus monkeys.

Shuang WangYun PengRongjuan WangShasha JiaoMin WangWei Jin HuangChao ShanWen JiangZepeng LiChunying GuBen ChenXue HuYanfeng YaoJuan MinHuajun ZhangYing ChenGe GaoPeipei TangGang LiAn WangLan WangJinchao ZhangShuo ChenXun GuiZhi-Ming YuanDatao Liu
Published in: Nature communications (2020)
Efficacious interventions are urgently needed for the treatment of COVID-19. Here, we report a monoclonal antibody (mAb), MW05, with SARS-CoV-2 neutralizing activity by disrupting the interaction of receptor binding domain (RBD) with angiotensin-converting enzyme 2 (ACE2) receptor. Crosslinking of Fc with FcγRIIB mediates antibody-dependent enhancement (ADE) activity by MW05. This activity is eliminated by introducing the LALA mutation to the Fc region (MW05/LALA). Potent prophylactic and therapeutic effects against SARS-CoV-2 are observed in rhesus monkeys. A single dose of MW05/LALA blocks infection of SARS-CoV-2 in prophylactic treatment and clears SARS-CoV-2 in three days in a therapeutic treatment setting. These results pave the way for the development of MW05/LALA as an antiviral strategy for COVID-19.
Keyphrases
  • sars cov
  • respiratory syndrome coronavirus
  • angiotensin converting enzyme
  • monoclonal antibody
  • coronavirus disease
  • zika virus
  • dna binding
  • anti inflammatory