Genome-wide CRISPR Screen Reveals RAB10 as a Synthetic Lethal Gene in Colorectal and Pancreatic Cancers Carrying SMAD4 Loss.

Hélène Erasimus Vanessa Kolnik Frédéric Lacroix Sukhvinder S Sidhu Stéphane D'Agostino Olivier Lemaitre Alexandre Rohaut Isabelle Sanchez Gilbert Thill Michel Didier Laurent Debussche Christophe Marcireau

Published in: Cancer research communications (2023)

This study identified and validated RAB10 as new synthetic lethal gene with SMAD4. This was achieved by conducting a whole-genome CRISPR screens in different colorectal and pancreatic cell lines. A future RAB10 inhibitors could correspond to a new therapeutic solution for patients with cancer with SMAD4 deletion.

Keyphrases

genome wide
epithelial mesenchymal transition
transforming growth factor
dna methylation
copy number
high throughput
signaling pathway