Synthesis and characterization of PLGA/HAP scaffolds with DNA-functionalised calcium phosphate nanoparticles for bone tissue engineering.
Viktoriya SokolovaKathrin KostkaK T ShalumonOleg PrymakJyh-Ping ChenMatthias EpplePublished in: Journal of materials science. Materials in medicine (2020)
Porous scaffolds of poly(lactide-co-glycolide) (PLGA; 85:15) and nano-hydroxyapatite (nHAP) were prepared by an emulsion-precipitation procedure from uniform PLGA-nHAP spheres (150-250 µm diameter). These spheres were then thermally sintered at 83 °C to porous scaffolds that can serve for bone tissue engineering or for bone substitution. The base materials PLGA and nHAP and the PLGA-nHAP scaffolds were extensively characterized by X-ray powder diffraction, infrared spectroscopy, thermogravimetry, differential scanning calorimetry, and scanning electron microscopy. The scaffold porosity was about 50 vol% as determined by relating mass and volume of the scaffolds, together with the computed density of the solid phase (PLGA-nHAP). The cultivation of HeLa cells demonstrated their high cytocompatibility. In combination with DNA-loaded calcium phosphate nanoparticles, they showed a good activity of gene transfection with enhanced green fluorescent protein (EGFP) as model protein. This is expected enhance bone growth around an implanted scaffold or inside a scaffold for tissue engineering.
Keyphrases
- tissue engineering
- bone regeneration
- electron microscopy
- drug delivery
- drug release
- bone mineral density
- high resolution
- circulating tumor
- bone loss
- soft tissue
- cancer therapy
- cell free
- postmenopausal women
- gene expression
- magnetic resonance imaging
- magnetic resonance
- genome wide
- protein protein
- quantum dots
- nucleic acid
- cell proliferation
- small molecule
- copy number
- oxidative stress
- optical coherence tomography