Structural effects of Diamond nanoparticles and Paclitaxel combination on calf thymus DNA.

The combination effects of nanodiamonds (NDs) and Paclitaxel (PTX) on the DNA structure were examined. The UV-Visible, steady-state and time-resolved fluorescence spectroscopy, CD, viscosity and zeta potential results showed that PTX + NDs could form a complex via groove binding mechanism. The values of binding constants, ΔG° and ΔH° and ΔS° values showed that PTX + NDs interact strongly with DNA and the hydrophobic force plays main role in this interaction. The ΔG25ο and Tm study indicated the instability of DNA in presence of PTX + NDs. This study demonstrated that NDs could enhance the effect of PTX on DNA structure as well as its affinity and binding to DNA.