Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI).
Robert TannenbergMartin PaulBettina RöderSantosh L GandeSridhar SreeramuluKrishna SaxenaChristian RichterHarald SchwalbeClaudia SwartMichael G WellerPublished in: Biosensors (2023)
Cardiac vascular diseases, especially acute myocardial infarction (AMI), are one of the leading causes of death worldwide. Therefore cardio-specific biomarkers such as cardiac troponin I (cTnI) play an essential role in the field of diagnostics. In order to enable rapid and accurate measurement of cTnI with the potential of online measurements, a chemiluminescence-based immunosensor is presented as a proof of concept. A flow cell was designed and combined with a sensitive CMOS camera allowing sensitive optical readout. In addition, a microfluidic setup was established, which achieved selective and quasi-online cTnI determination within ten minutes. The sensor was tested with recombinant cTnI in phosphate buffer and demonstrated cTnI measurements in the concentration range of 2-25 µg/L. With the optimized system, a limit of detection (LoD) of 0.6 µg/L (23 pmol/L) was achieved. Furthermore, the selectivity of the immunosensor was investigated with other recombinant proteins, such as cTnT, and cTnC, at a level of 16 µg/L. No cross-reactivity could be observed. Measurements with diluted blood plasma and serum resulted in an LoD of 60 µg/L (2.4 nmol/L) and 70 µg/L (2.9 nmol/L), respectively.
Keyphrases
- sensitive detection
- label free
- acute myocardial infarction
- loop mediated isothermal amplification
- molecularly imprinted
- quantum dots
- social media
- left ventricular
- high resolution
- high speed
- healthcare
- percutaneous coronary intervention
- stem cells
- atomic force microscopy
- coronary artery disease
- human health
- acute coronary syndrome
- convolutional neural network
- solid state