A red-NIR fluorescent dye detecting nuclear DNA G-quadruplexes: in vitro analysis and cell imaging.
Filippo DoriaMatteo NadaiM ZuffoRosalba PerroneMauro FrecceroS N RichterPublished in: Chemical communications (Cambridge, England) (2018)
Aggregation, red-NIR emission and light-up upon nucleic acid G-quadruplex binding have been investigated for a prototype core-extended naphthalene diimide, which is capable of fast cellular entry and nucleolar localization. Both high-level colocalization with an anti-G-quadruplex antibody and nucleolin displacement reveal that the compound targets and thus makes visible nuclear DNA G-quadruplexes.
Keyphrases
- nucleic acid
- single cell
- fluorescence imaging
- photodynamic therapy
- circulating tumor
- fluorescent probe
- drug release
- living cells
- high resolution
- cell free
- single molecule
- cell therapy
- quantum dots
- genome wide
- stem cells
- gene expression
- atomic force microscopy
- mesenchymal stem cells
- dna binding
- transcription factor
- high speed