Measurement of gene amplifications related to drug resistance in Plasmodium falciparum using droplet digital PCR.

Suttipat SrisuthamKanokon SuwannasinRungniran SugaramArjen M DondorpMallika Imwong

Published in: Malaria journal (2021)

The developed ddPCR assays are simple, accurate, precise and cost-effective tools for detection of the CNVs in the pfmdr1, pfplasmepsin2 and pfgch1 genes of P. falciparum. The ddPCR assay is a useful additional tool for the surveillance of anti-malarial drug resistance.

Keyphrases

plasmodium falciparum
high throughput
genome wide
genome wide identification
real time pcr
public health
single cell
genome wide analysis
copy number
high resolution
loop mediated isothermal amplification
label free
transcription factor
bioinformatics analysis
mass spectrometry
sensitive detection