Combined tumor genomic profiling and exome sequencing in a breast cancer family implicates ATM in tumorigenesis: A proof of principle study.
Virginie BubienFrançoise BonnetJennifer Dupiot-ChironEmmanuelle Barouk-SimonetNatalie JonesAurélien de ReyniesGaëtan MacGroganNicolas SevenetEric LetouzéMichel LongyPublished in: Genes, chromosomes & cancer (2017)
Familial breast cancers (BCs) account for 10%-20% of all diagnosed BCs, yet only 20% of such tumors arise in the context of a germline mutation in known tumor suppressor genes such as BRCA1 or BRCA2. The vast genetic heterogeneity which characterizes non BRCA1 and non BRCA2 (or BRCAx) families makes grouped studies impossible to perform. Next generation sequencing techniques, however, allow individual families to be studied to identify rare and or private mutations but the high number of genetic variants identified need to be sorted using pathogenicity or recurrence criteria. An additional sorting criterion may be represented by the identification of candidate regions defined by tumor genomic rearrangements. Indeed, comparative genomic hybridization (CGH) using single nucleotide polymorphism (SNP) arrays allows the detection of conserved ancestral haplotypes within recurrent regions of loss of heterozygosity, common to several familial tumors, which can highlight genomic loci harboring a germline mutation in cancer predisposition genes. The combination of both exome sequencing and SNP array-CGH for a series of familial BC revealed a germline ATM mutation associated with a loss of the wild-type allele in two BC from a BRCAx family. The analysis of additional breast tumors from ten BC families in which a germline ATM mutation had been identified revealed a high frequency of wild-type allele loss. This result argues strongly in favor of the involvement of ATM in these tumors as a tumor suppressor gene and confirms that germline ATM mutations are involved in a subset of familial BC.
Keyphrases
- copy number
- dna repair
- genome wide
- wild type
- single cell
- dna damage
- dna methylation
- high frequency
- dna damage response
- early onset
- breast cancer risk
- transcranial magnetic stimulation
- healthcare
- high throughput
- oxidative stress
- high density
- transcription factor
- papillary thyroid
- genome wide identification
- cystic fibrosis
- mass spectrometry
- biofilm formation
- free survival
- circulating tumor
- lymph node metastasis
- cell free
- loop mediated isothermal amplification