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A foliar pigment-based bioassay for interrogating chloroplast signalling revealed that carotenoid isomerisation regulates chlorophyll abundance.

Namraj DhamiBarry James PogsonDavid T TissueChristopher Ian Cazzonelli
Published in: Plant methods (2022)
The Arabidopsis foliar pigment-based bioassay can be used to differentiate signalling events elicited by environmental change, chemical treatment, and/or genetic perturbation, and determine how they control chloroplast biogenesis and chlorophyll biosynthesis. Genetic perturbations that impaired xanthophyll biosynthesis and/or carotenoid catabolism did not affect chlorophyll biosynthesis. The lack of CAROTENOID ISOMERISATION reduced chlorophyll accumulation, but not phytoene biosynthesis in young leaves of Arabidopsis plants growing under a long photoperiod. Findings generated using the newly customised foliar pigment-based bioassay implicate that carotenoid isomerase activity and NFZ-induced inhibition of PDS activity elicit different signalling pathways to control chlorophyll homeostasis in young leaves of Arabidopsis.
Keyphrases
  • cell wall
  • energy transfer
  • transcription factor
  • water soluble
  • genome wide
  • middle aged
  • copy number
  • single cell
  • diabetic rats
  • plant growth
  • combination therapy
  • oxidative stress
  • stress induced