Web-based LinRegPCR: application for the visualization and analysis of (RT)-qPCR amplification and melting data.

Andreas UntergasserJan M RuijterVladimir BenesMaurice J B van den Hoff

Published in: BMC bioinformatics (2021)

The combined implementation of these analyses in the newly developed web-based LinRegPCR ( https://www.gear-genomics.com/rdml-tools/ ) is platform independent and much faster than the original Windows-based versions of the LinRegPCR program. Moreover, web-based LinRegPCR includes a novel statistical outlier detection and the combination of amplification and melting curve analyses allows direct validation of the amplification product and reporting of reactions that amplify artefacts.

Keyphrases

nucleic acid
label free
high resolution
quality improvement
healthcare
primary care
high throughput
single cell
big data
electronic health record
emergency department
loop mediated isothermal amplification
mass spectrometry
machine learning
quantum dots