Photocatalytic Quantum Dot-Armed Bacteriophage for Combating Drug-Resistant Bacterial Infection.
Lei WangXin FanMercedes Gonzalez MorenoTamta TkhilaishviliWeijie DuXianlong ZhangChuanxiong NieAndrej TrampuzRainer HaagPublished in: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2022)
Multidrug-resistant (MDR) bacterial infection is one of the greatest challenges to public health, a crisis demanding the next generation of highly effective antibacterial agents to specifically target MDR bacteria. Herein, a novel photocatalytic quantum dot (QD)-armed bacteriophage (QD@Phage) is reported for combating green fluorescent protein-expressing Pseudomonas aeruginosa (GFP-P. aeruginosa) infection. The proposed QD@Phage nanosystem not only specifically binds to the host GFP-P. aeruginosa while preserving the infectivity of the phage itself, but also shows a superior capacity for synergistic bacterial killing by phage and by the photocatalytic localized reactive oxygen species (ROS) generated from anchored QD components. Notably, this highly targeted QD@Phage nanosystem achieves robust in vitro antibacterial elimination for both planktonic (over 99.9%) and biofilm (over 99%) modes of growth. In a mouse wound infection model, this system also shows remarkable activity in eliminating the wound infection and promoting its recovery. These results demonstrate that the novel QD@Phage nanosystem can diversify the existing pool of antibacterial agents and inspire the development of promising therapeutic strategies against MDR bacterial infection.
Keyphrases
- pseudomonas aeruginosa
- multidrug resistant
- drug resistant
- public health
- acinetobacter baumannii
- cystic fibrosis
- reactive oxygen species
- gram negative
- biofilm formation
- staphylococcus aureus
- dna damage
- oxidative stress
- klebsiella pneumoniae
- wound healing
- cancer therapy
- gold nanoparticles
- cell death
- small molecule
- quantum dots
- reduced graphene oxide
- binding protein
- living cells