Proteome analysis of Phytomonas serpens, a phytoparasite of medical interest.
Agenor de Castro Moreira Dos Santos JúniorCarlos André Ornelas RicartArthur Henriques PontesWagner FontesAgnelo Rodrigues de SouzaMariana Souza CastroMarcelo Valle de SousaBeatriz Dolabela de LimaPublished in: PloS one (2018)
The protozoan Phytomonas serpens (class Kinetoplastea) is an important phytoparasite that has gained medical importance due to its similarities to Trypanosoma cruzi, the etiological agent of Chagas disease. The present work describes the first proteome analysis of P. serpens. The parasite was separated into cytosolic and high density organelle fractions, which, together with total cell extract, were subjected to LC-MS/MS analyses. Protein identification was conducted using a comprehensive database composed of genome sequences of other related kinetoplastids. A total of 1,540 protein groups were identified among the three sample fractions. Sequences from Phytomonas sp. in the database allowed the highest number of identifications, with T. cruzi and T. brucei the human pathogens providing the greatest contribution to the identifications. Based on the proteomics data obtained, we proposed a central metabolic map of P. serpens, which includes all enzymes of the citric acid cycle. Data also revealed a new range of proteins possibly responsible for immunological cross-reactivity between P. serpens and T. cruzi.
Keyphrases
- high density
- trypanosoma cruzi
- single cell
- healthcare
- electronic health record
- endothelial cells
- big data
- protein protein
- oxidative stress
- amino acid
- emergency department
- genome wide
- cell therapy
- binding protein
- gene expression
- induced pluripotent stem cells
- mesenchymal stem cells
- machine learning
- gram negative
- anti inflammatory
- bioinformatics analysis