Login / Signup

Functional selectivity of insulin receptor revealed by aptamer-trapped receptor structures.

Junhong KimNa-Oh YunnMangeun ParkJihan KimSeongeun ParkYoojoong KimJeongeun NohSung Ho RyuYunje Cho
Published in: Nature communications (2022)
Activation of insulin receptor (IR) initiates a cascade of conformational changes and autophosphorylation events. Herein, we determined three structures of IR trapped by aptamers using cryo-electron microscopy. The A62 agonist aptamer selectively activates metabolic signaling. In the absence of insulin, the two A62 aptamer agonists of IR adopt an insulin-accessible arrowhead conformation by mimicking site-1/site-2' insulin coordination. Insulin binding at one site triggers conformational changes in one protomer, but this movement is blocked in the other protomer by A62 at the opposite site. A62 binding captures two unique conformations of IR with a similar stalk arrangement, which underlie Tyr1150 mono-phosphorylation (m-pY1150) and selective activation for metabolic signaling. The A43 aptamer, a positive allosteric modulator, binds at the opposite side of the insulin-binding module, and stabilizes the single insulin-bound IR structure that brings two FnIII-3 regions into closer proximity for full activation. Our results suggest that spatial proximity of the two FnIII-3 ends is important for m-pY1150, but multi-phosphorylation of IR requires additional conformational rearrangement of intracellular domains mediated by coordination between extracellular and transmembrane domains.
Keyphrases
  • type diabetes
  • glycemic control
  • gold nanoparticles
  • molecular dynamics simulations
  • sensitive detection
  • high resolution
  • electron microscopy
  • small molecule
  • dna binding
  • quantum dots
  • reactive oxygen species