A novel multi-functional fluorescence probe HMIC based on hydrazide Schiff base has been successfully synthesized and characterized. It can distinguish Al 3+ /Zn 2+ /Cd 2+ in ethanol, in which fluorescence emission with different colors (blue for Al 3+ , orange for Zn 2+ , and green for Cd 2+ ) were presented. The limits of detection of HMIC towards three ions were calculated from the titration curve as 7.70 × 10 - 9 M, 4.64 × 10 - 9 M, and 1.35 × 10 - 8 M, respectively. The structures of HMIC and its complexes were investigated using UV-Vis spectra, Job's plot, infrared spectra, mass spectrometry, 1 H-NMR and DFT calculations. Practical application studies have also demonstrated that HMIC can be applied to real samples with a low impact of potential interferents. Cytotoxicity and cellular imaging assays have shown that HMIC has good cellular permeability and potential antitumor effects. Interestingly, HMIC can image Al 3+ , Zn 2+ and Cd 2+ in the cells with different fluorescence signals.
Keyphrases
- high resolution
- single molecule
- density functional theory
- heavy metals
- mass spectrometry
- quantum dots
- nk cells
- induced apoptosis
- magnetic resonance
- living cells
- high throughput
- endothelial cells
- deep learning
- loop mediated isothermal amplification
- machine learning
- label free
- climate change
- aqueous solution
- liquid chromatography
- cell cycle arrest
- atomic force microscopy
- solid state
- depressive symptoms
- endoplasmic reticulum stress
- case control
- ms ms
- fluorescent probe
- crystal structure
- gas chromatography
- light emitting
- capillary electrophoresis