UV-assisted synthesis of ultra-small GO-Austar for efficient PTT therapeutic architectonic construction.
Ang GaoLijia PeiGuan LiuYunsheng ChenAmin ZhangDa-Xiang CuiPublished in: RSC advances (2024)
Conventional Au nanomaterial synthesis typically necessitates the involvement of extensive surfactants and reducing agents, leading to a certain amount of chemical waste and biological toxicity. In this study, we innovatively employed ultra-small graphene oxide as a reducing agent and surfactant for the in situ generation of small Au nanoparticles under ultraviolet irradiation (UV) at ambient conditions. After ultra-small GO-Au seeds were successfully synthesized, we fabricated small star-like Au nanoparticles on the surface of GO, in which GO effectively prevented Austar from aggregation. To further use GO-Austar for cancer PTT therapy, through the modification of reduced human serum albumin-folic acid conjugate (rHSA-FA) and loading IR780, the final probe GO-Austar@rHSA-FA@IR780 was prepared. The prepared probe showed excellent biocompatibility and superb phototoxicity towards MGC-803 cells in vitro . In vivo , the final probe dramatically increased tumor temperature up to 58.6 °C after 5 minutes of irradiation by an 808 nm laser, significantly inhibiting tumor growth and nearly eradicating subcutaneous tumors in mice. This research provides a novel and simple method for the synthesis of GO-Au nanocomposites, showcasing significant potential in biological applications.
Keyphrases
- sensitive detection
- reduced graphene oxide
- quantum dots
- high resolution
- living cells
- oxidative stress
- induced apoptosis
- squamous cell carcinoma
- signaling pathway
- visible light
- type diabetes
- air pollution
- heavy metals
- metabolic syndrome
- young adults
- stem cells
- insulin resistance
- gold nanoparticles
- mesenchymal stem cells
- risk assessment
- skeletal muscle
- mass spectrometry
- radiation induced
- single molecule
- human health
- fluorescent probe
- lymph node metastasis
- high fat diet induced