Single Cell Analysis of Proteoforms.
Pei SuMichael A R HollasFatma Ayaloglu ButunKanchustambham VijayalakshmiStanislav S RubakhinNamrata RamaniJoseph B GreerBryan P EarlyRyan T FellersMichael A CaldwellJonathan V SweedlerJared O KafaderMichael P SnyderPublished in: Journal of proteome research (2024)
We introduce single cell Proteoform imaging Mass Spectrometry ( scPiMS ), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells dropcast onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enable a scalable approach to single cell proteomics. This new scPiMS platform addresses the throughput bottleneck in single cell proteomics and boosts the cell processing rate by several fold while accessing protein composition with higher coverage.
Keyphrases
- single cell
- mass spectrometry
- rna seq
- high throughput
- liquid chromatography
- high resolution
- label free
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- induced apoptosis
- loop mediated isothermal amplification
- healthcare
- cell cycle arrest
- multiple sclerosis
- ionic liquid
- tandem mass spectrometry
- mesenchymal stem cells
- stem cells
- solid phase extraction