Characterization of Heat-labile Uracil-DNA Glycosylase from Oncorhynchus mykiss and its Application for Carry-over Contamination Control in RT-qPCR.

Qingyuan HuangYaqi ZhangWenhao HuKeqi ChenJian ZhangZhidan Luo Chen Lu

Published in: Protein and peptide letters (2024)

We successfully identified and recombinantly expressed a novel HL-UDG with an inactivation temperature of 40°C. It is suitable for eliminating carry-over contamination in one-step RT-qPCR.

Keyphrases

risk assessment
drinking water
health risk
human health
circulating tumor
dna repair
single molecule
cell free
heat stress
heavy metals
nucleic acid