A Droplet-Based Microfluidic Platform for High-Throughput Culturing of Yeast Cells in Various Conditions.
Min-Chieh YuYung-Shin SunPublished in: Micromachines (2024)
Yeast plays a significant role in a variety of fields. In particular, it is extensively used as a model organism in genetics and cellular biology studies, and is employed in the production of vaccines, pharmaceuticals, and biofuels. Traditional "bulk"-based studies on yeast growth often overlook cellular variability, emphasizing the need for single-cell analysis. Micro-droplets, tiny liquid droplets with high surface-area-to-volume ratios, offer a promising platform for investigating single or a small number of cells, allowing precise control and monitoring of individual cell behaviors. Microfluidic devices, which facilitate the generation of micro-droplets, are advantageous due to their reduced volume requirements and ability to mimic in vivo micro-environments. This study introduces a custom-designed microfluidic device to encapsulate yeasts in micro-droplets under various conditions in a parallel manner. The results reveal that optimal glucose concentrations promoted yeast growth while cycloheximide and Cu 2+ ions inhibited it. This platform enhances yeast cultivation strategies and holds potential for high-throughput single-cell investigations in more complex organisms.
Keyphrases
- risk assessment
- high throughput
- single cell
- saccharomyces cerevisiae
- rna seq
- induced apoptosis
- cell cycle arrest
- cell wall
- oxidative stress
- stem cells
- cell death
- endoplasmic reticulum stress
- signaling pathway
- metabolic syndrome
- bone marrow
- case control
- insulin resistance
- blood pressure
- dna methylation
- mesenchymal stem cells
- climate change
- blood glucose
- circulating tumor cells