Synthesis of Two-Photon Active Tricomponent Fluorescent Probe for Distinguishment of Biotin Receptor Positive and Negative Cells and Imaging 3D-Spheroid.

Published in: Organic letters (2018)

A fluorescence microscopy-based distinguishment between biotin receptor (BiR) positive and negative cell lines via receptor-mediated endocytosis has been demonstrated. A water-soluble, three-component, two-photon (2P) active solvatofluorochromic probe has been designed and synthesized. The applicability of the probe for 2P microscopy and 3D-spheroid was also assessed.

Keyphrases

living cells
single molecule
fluorescent probe
water soluble
high resolution
induced apoptosis
cell cycle arrest
high speed
high throughput
optical coherence tomography
binding protein
cell death
oxidative stress
mass spectrometry
quantum dots
endoplasmic reticulum stress
photodynamic therapy
label free