Methodological approaches to optimize multiplex oral fluid SARS-CoV-2 IgG assay performance and correlation with serologic and neutralizing antibody responses.

Nora Pisanic Annukka A R AntarKate KruczynskiMagdielis Gregory RiveraSantosh DhakalKristoffer SpicerPranay R RandadAndrew S Pekosz Sabra L KleinMichael J BetenbaughBarbara DetrickWilliam ClarkeDavid L ThomasYukari C ManabeChristopher D Heaney

Published in: medRxiv : the preprint server for health sciences (2022)

A salivary SARS-CoV-2 IgG MIA produced consistently high Se (>98.8%) and Sp (>99.1%) across two bead coupling scales and correlations with nAb responses that were similar to blood-based SARS-CoV-2 IgG ELISA data. This non-invasive salivary SARS-CoV-2 IgG MIA could increase engagement of vulnerable populations and improve broad understanding of humoral immunity (kinetics and gaps) within the evolving context of booster vaccination, viral variants and waning immunity.

Keyphrases

sars cov
respiratory syndrome coronavirus
high throughput
immune response
electronic health record
copy number
advanced non small cell lung cancer
big data
machine learning
dna methylation
room temperature
monoclonal antibody
epidermal growth factor receptor
genome wide
tyrosine kinase