COInr and mkCOInr: Building and customizing a non-redundant barcoding reference database from BOLD and NCBI using a semi-automated pipeline.

Reference databases with a wide taxonomic coverage are strongly needed in many fields of biology, most particularly for the taxonomic assignment of metabarcoding sequences. Therefore, it is fundamental to be able to access and pool data from different primary databases. The COInr database is a freely available, easy-to-access database of COI reference sequences extracted from the BOLD and NCBI nucleotide databases. It is a comprehensive database: not limited to a taxon, a gene region, or a taxonomic rank; therefore, it is a good starting point for creating custom databases. Sequences are dereplicated between databases and within taxa. Each taxon has a unique taxonomic Identifier (taxID), fundamental to avoid ambiguous associations of homonyms and synonyms in the source database. TaxIDs form a coherent hierarchical system fully compatible with the NCBI taxIDs allowing to create their full or ranked linages. The mkCOInr tool is a series of Perl scripts designed to download sequences from BOLD and NCBI, to build the COInr database and to customize it according to the users' needs. It is possible to select or eliminate sequences for a list of taxa, select a specific gene region, select for minimum taxonomic resolution, add new custom sequences, and format the database for BLAST, VTAM, QIIME, RDP classifier. This is a semi-automated pipeline using command lines in a Linux environment. The COInr database can be downloaded from https://doi.org/10.5281/zenodo.6555985 and mkCOInr and its full documentation is available at https://github.com/meglecz/mkCOInr.