Validation of an apoptosis assay for extracorporeal photopheresis.

Viola HaehnelFrauke DormannKatharina KronenbergJames A HutchinsonRalph BurkhardtNorbert Ahrens

Published in: Transfusion medicine (Oxford, England) (2021)

The results of this validation study show that the assay is GMP-compliant and that its reliability, accuracy and precision are acceptable. While pre-analytical stability of the cells was compatible with on-site procedures, our analytical stability data indicate that this assay is not suited for batch mode analysis of ECP products.

Keyphrases

cell cycle arrest
high throughput
induced apoptosis
cell death
endoplasmic reticulum stress
oxidative stress
liquid chromatography
pi k akt
signaling pathway
mass spectrometry
big data
biofilm formation
staphylococcus aureus
machine learning
cystic fibrosis
single cell
data analysis