Ratiometric analysis of reversible thia-Michael reactions using nitrile-tagged molecules by Raman microscopy.

Hiroyuki Yamakoshi Daiki Shibata Kazuki Bando Shinji Kajimoto Aki Kohyama Syusuke Egoshi Kosuke Dodo Yoshiharu Iwabuchi Mikiko Sodeoka Katsumasa Fujita Takakazu Nakabayashi

Published in: Chemical communications (Cambridge, England) (2023)

Ratiometric Raman analysis of reversible thia-Michael reactions was achieved using α-cyanoacrylic acid (αCNA) derivatives. Among αCNAs, the smallest derivative, ThioRas (molecular weight: 167 g mol -1 ), and its glutathione adduct were simultaneously detected in various subcellular locations using Raman microscopy.

Keyphrases

label free
living cells
single molecule
fluorescent probe
quantum dots
high resolution
sensitive detection
high speed
raman spectroscopy
hydrogen peroxide
high throughput
optical coherence tomography
energy transfer