Synergistic Effect Evaluation of Magnetotherapy and a Cationic-Magnetic Nanocomposite Loaded with Doxorubicin for Targeted Drug Delivery to Breast Adenocarcinoma.
Alireza NaghaviyanHamid Hashemi-MoghaddamSaeed ZavarehMahboobeh Ebrahimi VerkianiAnja MeullerPublished in: Molecular pharmaceutics (2022)
This work investigates the synergistic effect of magnetotherapy and a novel cationic-magnetic drug delivery system on inhibiting breast cancer cell growth and other tissues. First, super-paramagnetic magnetite (Fe 3 O 4 ) nanoparticles were coated with doxorubicin-imprinted poly(methacrylic acid- co -diallyl dimethylammonium chloride) [Fe 3 O 4 /poly(MAA-DDA)]. The cationic-magnetic nanocomposite (CMC) was characterized using XRD, FT-IR, VSM, TGA, TEM, FESEM, EDS, DLS, and BET. In vitro analyses, including drug release kinetics, cytotoxicity, and hemolytic assays, confirmed this novel CMC's good drug release profile and biocompatibility. Finally, in vivo experiments on BALB/c mice were designed to evaluate the synergistic effect of magnetotherapy on targeted drug delivery using the CMC. In vivo fluorescence imaging evaluated the drug distribution in different tissues of mice. Tumor volume evaluation demonstrated the efficiency of the CMC and magnetotherapy in preventing tumor growth; the two techniques significantly reduced tumor volume. Histopathological analysis proved that applying magnetotherapy in conjunction with the cationic-magnetic drug delivery system significantly prevented tumor cell proliferation and increased apoptosis with limited impact on other tissues. Also, Dox and Fe concentrations in different tissues confirmed the efficient drug delivery to tumor cells.
Keyphrases
- drug delivery
- drug release
- cancer therapy
- molecularly imprinted
- gene expression
- fluorescence imaging
- cell proliferation
- solid phase extraction
- photodynamic therapy
- high fat diet induced
- emergency department
- cell death
- reduced graphene oxide
- quantum dots
- aqueous solution
- endoplasmic reticulum stress
- radiation therapy
- type diabetes
- metabolic syndrome
- cell cycle
- mass spectrometry
- adipose tissue
- skeletal muscle
- insulin resistance
- pi k akt
- clinical evaluation