Synthesis of NNN Chiral Ruthenium Complexes and Their Cytotoxicity Studies.
Pran Gobinda NandiPraveen Kumar JadiKanu DasSiriyara Jagannatha PrathapaBiman B MandalAkshai KumarPublished in: Inorganic chemistry (2021)
The synthesis and characterization of chiral pincer-ruthenium complexes of the type (R2NNN)RuCl2 (PPh3) (R = 3-methylbutyl and 3,3-dimethylbutyl) is reported here. The cytotoxicity studies of these complexes were studied and compared with the corresponding activity of achiral complexes. The cytotoxic effect of pincer-ruthenium complexes on human dermal fibroblasts and human tongue carcinoma cells assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay displayed an inhibition of normal and cancer cell growth in a dose-dependent manner. Intracellular reactive oxygen species (ROS) level measurement, lactate dehydrogenase assay, DNA fragmentation, and necrosis studies revealed that treatment with pincer-ruthenium complexes induced a redox imbalance in SAS cells by upregulating ROS generation and caused necrotic cell death by disrupting the cellular membrane integrity.
Keyphrases
- reactive oxygen species
- cell death
- endothelial cells
- cell cycle arrest
- case control
- high throughput
- dna damage
- cell proliferation
- high glucose
- papillary thyroid
- single molecule
- cell free
- signaling pathway
- endoplasmic reticulum stress
- diabetic rats
- lymph node metastasis
- stress induced
- pi k akt
- extracellular matrix
- electron transfer