Polarity-Driven Two-Photon Fluorescent Probe for Monitoring the Perturbation in Lipid Droplet Levels during Mitochondrial Dysfunction and Acute Pancreatitis.
Shayeri BiswasMousumi BaruahAnushree ShilSourav SarkarMudassar AliAnimesh SamantaSankarprasad BhuniyaPublished in: ACS sensors (2023)
Lipid droplets (LDs) act as an energy reservoir in cancer cells; on the other hand, mitochondria are hyperactive to fulfill the energy demand to accelerate cell proliferation. We are interested in unfolding the relationship between the cellular energy reservoir and energy producer through fluorescence labeling. Thus, a dual organelle-targeted fluorescent probe MLD-1 has been rationally developed. It visualized the crosstalk between mitochondrial dysfunction and the fluctuation of LDs in live cells. Its two-photon ability allowed us to acquire deep tissue images. For the first time, we have shown that the probe has the ability to track the accumulation of LDs in different mouse organs during pancreatic inflammation. MLD-1 , being a selectively polarity-driven, chemo- and photostable LD probe, may offer great possibilities for studying LD-associated biology in due course.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- cell proliferation
- induced apoptosis
- oxidative stress
- cell death
- cancer therapy
- deep learning
- cell cycle arrest
- squamous cell carcinoma
- photodynamic therapy
- single cell
- radiation therapy
- convolutional neural network
- machine learning
- endoplasmic reticulum stress
- drug delivery
- pi k akt
- rectal cancer
- endoplasmic reticulum