Notch signaling in the dynamics of perivascular stem cells and their niches.
Pierfrancesco PagellaLaura de Vargas RoditiBernd StadlingerAndreas E MoorThimios A MitsiadisPublished in: Stem cells translational medicine (2021)
The Notch signaling pathway is a fundamental regulator of cell fate determination in homeostasis and regeneration. In this work, we aimed to determine how Notch signaling mediates the interactions between perivascular stem cells and their niches in human dental mesenchymal tissues, both in homeostatic and regenerative conditions. By single cell RNA sequencing analysis, we showed that perivascular cells across the dental pulp and periodontal human tissues all express NOTCH3, and that these cells are important for the response to traumatic injuries in vivo in a transgenic mouse model. We further showed that the behavior of perivascular NOTCH3-expressing stem cells could be modulated by cellular and molecular cues deriving from their microenvironments. Taken together, the present studies, reinforced by single-cell analysis, reveal the pivotal importance of Notch signaling in the crosstalk between perivascular stem cells and their niches in tissue homeostasis and regeneration.
Keyphrases
- stem cells
- single cell
- induced apoptosis
- rna seq
- signaling pathway
- endothelial cells
- mouse model
- cell cycle arrest
- cell therapy
- cell proliferation
- cell fate
- high throughput
- gene expression
- spinal cord injury
- induced pluripotent stem cells
- endoplasmic reticulum stress
- pi k akt
- transcription factor
- genome wide
- dna methylation
- epithelial mesenchymal transition
- oral health
- solid phase extraction