A Monovalent Fab Affinity-Capture and Elution Bridging Immunoassay Overcomes Rheumatoid Factor Interference while Accurately Detecting Antidrug Antibodies.

Boris CalderónNichole A ReynoldsCraig A SwearingenQuincy L CarterNicoletta BiviJeff W CramerCourtney L AlexanderRobert W SiegelRobert J Konrad

Published in: The journal of applied laboratory medicine (2023)

RF interfered significantly in traditional ACE and ACE-Bridge assays. Implementation of a novel monovalent Fab ACE-Bridge assay overcame RF interference. The use of monovalent Fab is recommended for immunogenicity assays when assessing ADA in RA patient samples.

Keyphrases

angiotensin converting enzyme
high throughput
angiotensin ii
primary care
healthcare
rheumatoid arthritis
case report
quality improvement
sensitive detection
single cell