Liquid chromatography-tandem mass spectrometry glycoproteomic study of porcine IgG and detection of subtypes.

Taylor BattellinoRaymond BacalaBaylie GigolykGideon OngMilan V TeraiyaHélène Perreault

Published in: Rapid communications in mass spectrometry : RCM (2021)

Proteomic analysis of porcine IgG is complicated by factors such as the presence of several subtypes, redundant heavy chain (HC) sequences in protein databases, and the lack of consistent cross-referencing between databases. Aligning and comparing HC sequences were necessary to eliminate redundancy. This study highlights the complexity of pig IgG and shows the importance of MS in proteomics and glycoproteomics.

Keyphrases

liquid chromatography tandem mass spectrometry
mass spectrometry
ms ms
label free
multiple sclerosis
simultaneous determination
high resolution
deep learning
loop mediated isothermal amplification
protein protein
liquid chromatography