Whole genome sequencing Mycobacterium tuberculosis directly from sputum identifies more genetic diversity than sequencing from culture.

Camus NimmoLiam P ShawRonan DoyleRachel WilliamsKayleen BrienCarrie BurgessJudith BreuerFrancois BallouxAlexander S Pym

Published in: BMC genomics (2019)

Culture-free M. tuberculosis whole genome sequencing detects more within-sample diversity than a leading culture-based method and may allow detection of mycobacteria that are not actively replicating.

Keyphrases

mycobacterium tuberculosis
genetic diversity
pulmonary tuberculosis
single cell
cystic fibrosis
genome wide
gene expression
hepatitis c virus
human immunodeficiency virus
antiretroviral therapy