A near-infrared fluorescent probe for detecting hydrazine metabolized from isoniazid in living cells.

Isoniazid is a drug for treating tuberculosis, but hydrazine (N 2 H 4 ), the major metabolite of isoniazid, can cause hepatotoxicity. Therefore, monitoring the content of N 2 H 4 in time is of great significance for studying the hepatotoxicity induced by isoniazid. In this study, a near-infrared fluorescent probe (BC-N) was designed and synthesized based on the specific reaction of acetyl ester with N 2 H 4 . BC-N exhibits excellent selectivity, sensitivity, and biocompatibility. In addition, BC-N is applied in the visualization of N 2 H 4 produced from isoniazid in living cells and is a potential tool for monitoring hepatotoxicity induced by isoniazid.