Characterization of Non-Cholesterol Sterols in Microglia Cell Membranes Using Targeted Mass Spectrometry.

Ilijana Begcevic BrkovicMadlen Reinicke Soroth CheyIngo BechmannUta Ceglarek

Published in: Cells (2023)

By applying our newly developed and validated analytical protocol, we show that the non-CH sterol concentration is about 38% of the total sterol content in microglia membrane microdomains. Further investigations must clarify how changes in the non-sterol composition influence membrane fluidity and cell signaling.

Keyphrases

mass spectrometry
single cell
inflammatory response
cell therapy
liquid chromatography
neuropathic pain
low density lipoprotein
spinal cord
stem cells
spinal cord injury
capillary electrophoresis