Phthalides Isolated from the Endolichenic Arthrinium sp. EL000127 Exhibits Antiangiogenic Activity.
Chathurika D B GamageKyungha LeeSo-Yeon ParkMücahit VarlıChang Wook LeeSeong-Min KimRui ZhouSultan PulatYi Yangİsa TaşJae-Seoun HurKyo-Bin KangHangun KimPublished in: ACS omega (2023)
Endolichenic fungi (ELF) produce specialized metabolites that have various medicinal properties. Inhibition of tumor angiogenesis efficaciously suppresses many types of cancer. This study aimed to discover novel antiangiogenic agents from specialized metabolite extracts of ELF strains isolated from Korean lichens. The EtOAc extracts of 51 ELF strains were subjected to a screening pipeline consisting of cell viability, scratch wound healing, and Transwell migration assays. The EtOAc extract of Arthrinium sp. EL000127 showed the most potent inhibitory activity against the chemotactic migration of human umbilical vein endothelial cells (HUVEC). Targeted isolation on the major LC-MS peaks exhibited a previously known phthalide, 3- O -methylcyclopolic acid ( 1 ), and two unknown analogues of 1 , 3- O -phenylethylcyclopolic acid ( 2 ) and 3- O - p -hydroxyphenylethylcyclopolic acid ( 3 ). The structures were characterized by MS and NMR analyses. All the isolates were acquired and applied to bioassays as racemates due to spontaneous racemization. Among the isolates, compound 3 effectively inhibits HUVEC motility by suppressing mRNA expressions of genes regulating epithelial cell survival and motility, which suggested that compound 3 is a potent antiangiogenic agent suitable for further exploration as a potential novel therapeutic against cancers.
Keyphrases
- endothelial cells
- wound healing
- escherichia coli
- ms ms
- high resolution
- palliative care
- anti inflammatory
- magnetic resonance
- biofilm formation
- signaling pathway
- genetic diversity
- oxidative stress
- mass spectrometry
- molecular docking
- vascular endothelial growth factor
- gene expression
- transcription factor
- cystic fibrosis
- climate change
- binding protein
- dna methylation
- structure activity relationship