Sulfur-doped graphitic carbon nitride nanosheets as a sensitive fluorescent probe for detecting environmental and intracellular Ag .
Lingling ZhengYangyang YanNing WangMingli LiShaomin ShuangWei BianMartin M F ChoiPublished in: Methods and applications in fluorescence (2022)
Silver is widely used in medical materials, photography, electronics and other industries as a precious metal. The large-scale industrial production of silver-containing products and liquid waste emissions aggravate the environmental pollution. Silver ion is one of the most toxic metal ions, causing pollution to the environment and damage to public health. Therefore, the efficient and sensitive detection of Ag + in the water environment is extremely important. Sulfur-doped carbon nitride nanosheets (SCN Ns) were prepared by melamine and thiourea via high-temperature calcination. The morphology, chemical composition and surface functional groups of the SCN Ns were characterized by SEM, TEM, XRD, XPS, and FT-IR. The fluorescence of SCN Ns was gradually quenched as the Ag + concentration increased. The detection limit for Ag + was as low as 0.28 nM. The quenching mechanism mainly is attributed to static quenching. In this paper, SCN Ns were used as the fluorescent probe for detecting Ag + . SCN Ns have successfully detected Ag + in different environmental aqueous samples and cells. Finally, SCN Ns were further applied to the visual quantitative detection of intracellular Ag + .
Keyphrases
- quantum dots
- sensitive detection
- visible light
- fluorescent probe
- energy transfer
- dengue virus
- living cells
- heavy metals
- loop mediated isothermal amplification
- public health
- human health
- highly efficient
- gold nanoparticles
- risk assessment
- life cycle
- healthcare
- particulate matter
- high temperature
- reactive oxygen species
- ionic liquid
- zika virus
- silver nanoparticles
- photodynamic therapy
- mass spectrometry
- cell death
- oxidative stress
- cell cycle arrest
- label free
- health risk assessment
- signaling pathway
- cell proliferation
- single molecule
- climate change
- endoplasmic reticulum stress
- drinking water
- municipal solid waste
- tandem mass spectrometry
- liquid chromatography