The difficulty of near-infrared (NIR) ratiometric detection imaging lies in the lack of high-efficiency NIR probes and the overlapping interference between two emission peaks. To achieve more accurate detection in living organisms, dual NIR-emissive luminescent nanoprobes were designed under the same excitation at 808 nm. The Er3+ ion-doped nanoparticles were employed as a reference with their fluorescence emission at 1525 nm. Meanwhile, a cyanine dye molecule (Cy925) was combined on the surface of nanoparticles as the ClO- recognition site with its NIR emission at 925 nm. The ratiometric nanoprobe relied on the ratio of aforementioned two separated NIR peaks ( I925nm/ I1525nm), featuring deeper imaging penetration depth and low autofluorescence. This nanoprobe was verified to be sensitive and highly selective to ClO- through photoluminescence titration. The in vitro detection experiment developed reasonable work curves, guaranteeing that we can detect the change in concentration of ClO- in mice limbs with arthritis through in vivo imaging experiments.
Keyphrases
- photodynamic therapy
- fluorescence imaging
- quantum dots
- fluorescent probe
- living cells
- energy transfer
- sensitive detection
- light emitting
- loop mediated isothermal amplification
- high resolution
- high efficiency
- drug release
- metal organic framework
- label free
- single molecule
- real time pcr
- adipose tissue
- highly efficient
- drug delivery
- high fat diet induced
- hydrogen peroxide
- skeletal muscle