Photolon Nanoporous Photoactive Material with Antibacterial Activity and Label-Free Noncontact Method for Free Radical Detection.
Igor BuzalewiczIwona HołowaczAnna K MatczukMateusz GuźniczakDominika SkrzelaMagdalena KarwańskaAlina WieliczkoKatarzyna KowalAgnieszka Ulatowska-JarżaPublished in: International journal of molecular sciences (2021)
The worldwide increase in bacterial resistance and healthcare-associated bacterial infections pose a serious threat to human health. The antimicrobial photodynamic method reveals the opportunity for a new therapeutic approach that is based on the limited delivery of photosensitizer from the material surface. Nanoporous inorganic-organic composites were obtained by entrapment of photosensitizer Photolon in polysiloxanes that was prepared by the sol-gel method. The material was characterized by its porosity, optical properties (fluorescence and absorbance), and laser-induced antimicrobial activity against Staphylococcus epidermidis , Staphylococcus aureus , Pseudomonas aeruginosa , and Escherichia coli . The permanent encapsulation of Photolon in the silica coating and the antimicrobial efficiency was confirmed by confocal microscope and digital holotomography. The generation of free radicals from nanoporous surfaces was proved by scanning Kelvin probe microscopy. For the first time, it was confirmed that Kelvin probe microscopy can be a label-free, noncontact alternative to other conventional methods based on fluorescence or chemiluminescence probes, etc. It was confirmed that the proposed photoactive coating enables the antibacterial photodynamic effect based on free radicals released from the surface of the coating. The highest bactericidal efficiency of the proposed coating was 87.16%. This coating can selectively limit the multiplication of bacterial cells, while protecting the environment and reducing the risk of surface contamination.
Keyphrases
- label free
- staphylococcus aureus
- biofilm formation
- human health
- pseudomonas aeruginosa
- single molecule
- escherichia coli
- risk assessment
- healthcare
- photodynamic therapy
- living cells
- induced apoptosis
- cancer therapy
- energy transfer
- high resolution
- quantum dots
- climate change
- small molecule
- methicillin resistant staphylococcus aureus
- signaling pathway
- drug delivery
- metal organic framework
- candida albicans
- heavy metals
- high throughput
- cell proliferation
- endoplasmic reticulum stress
- wound healing
- fluorescent probe
- silver nanoparticles
- simultaneous determination
- oxidative stress