Nanoscale characterization of drug-induced microtubule filament dysfunction using super-resolution microscopy.

Ashley M RozarioSam DuwéCade ElliottRiley B HargreavesGregory W MoseleyPeter DedeckerDonna R WhelanToby D M Bell

Published in: BMC biology (2021)

Our results support the emerging hypothesis that microtubule-interacting drugs induce non-mitotic effects in cells, and establish a multi-modal imaging assay for detecting and measuring nanoscale microtubule dysfunction. The sub-diffraction visualization of these less severe precursor perturbations compared to the established antimitotic effects of microtubule-interacting drugs offers potential for improved understanding and design of anticancer agents.

Keyphrases

drug induced
liver injury
high resolution
induced apoptosis
high throughput
oxidative stress
atomic force microscopy
protein kinase
cell cycle arrest
adverse drug
cell cycle
early onset
risk assessment
photodynamic therapy
cell proliferation
crystal structure
climate change