A systematic study on the effect of protonation and deuteration on electron spin T m / T 2 in a cellular context.

In recent years, DEER experiments in pulsed EPR have garnered interest for their precise distance distribution insights in cellular and buffer setups. These measurements linked to electron spin T m / T 2 values of the labelled sample are impacted by the cellular environment being fully protonated or deuterated, as demonstrated in the present study.