A novel method for transforming the thermophilic bacterium Geobacillus kaustophilus.

Megumi MiyanoKosei TanakaShu IshikawaKotaro MoriAndrés Miguel-ArribasWilfried J J MeijerKen-Ichi Yoshida

Published in: Microbial cell factories (2018)

We describe a novel method of plasmid mobilization into G. kaustophilus recipient from B. subtilis donor depending on the helper function of pLS20cat, which enables simple, rapid, and easy transformation of the thermophilic Gram-positive bacterium.

Keyphrases

anaerobic digestion
escherichia coli
regulatory t cells
crispr cas
immune response
sensitive detection