Proteomic Profiling of Messenger Ribonucleoproteins in Mouse Tissues Based on Formaldehyde Cross-Linking.
Jiankun WangJialin LiuRongbing HuangTianyu ChuQi TangXing ChenPublished in: Journal of proteome research (2024)
Messenger ribonucleoprotein particles (mRNPs) are vital for tissue-specific gene expression via mediating posttranscriptional regulations. However, proteomic profiling of proteins in mRNPs, i.e., mRNA-associated proteins (mRAPs), has been challenging at the tissue level. Herein, we report the development of formaldehyde cross-linking-based mRNA-associated protein profiling (FAXRAP), a chemical strategy that enables the identification of mRAPs in both cultured cells and intact mouse organs. Applying FAXRAP, tissue-specific mRAPs were systematically profiled in the mouse liver, kidney, heart, and brain. Furthermore, brain mRAPs in Parkinson's disease (PD) mouse model were investigated, which revealed a global decrease of mRNP assembly in the brain of mice with PD. We envision that FAXRAP will facilitate uncovering the posttranscriptional regulation networks in various biological systems.
Keyphrases
- gene expression
- single cell
- resting state
- white matter
- mouse model
- functional connectivity
- induced apoptosis
- heart failure
- dna methylation
- cerebral ischemia
- room temperature
- binding protein
- cell cycle arrest
- multiple sclerosis
- signaling pathway
- adipose tissue
- label free
- skeletal muscle
- cell proliferation
- pi k akt
- subarachnoid hemorrhage